Composite

Part:BBa_K1955006:Design

Designed by: Justine Hsu   Group: iGEM16_CGU_Taiwan   (2016-10-14)


Leishmania 5 prime UTR-HYG-OVA-3 prime UTR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1458
    Illegal BamHI site found at 3562
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 2689


Design Notes

The detailed design of the sequence is documented in each part.


Source

Composite of the Leishmania 5 prime UTR-HYG, ovalbumin and 3 prime UTR. The Leish-5'UTR and 3'UTR sequence originally came from Leishmania genome and is acquired from GenBank. The Hygromycin resistant gene and ovalbumin sequences are acquired from GenBank.

References